JRC GMO-Amplicons | EURL GMFF

Record ID:	OR778394.1
Database:	nt	Go to original record
Description:	Damrongia clarkeana chloroplast, complete genome.
Taxon:	Damrongia clarkeana
Original size:	153142 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47829	48382	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa gggtttaaag
151   ggcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgtgttggta gaggaattaa aacttcagaa aggatgaagg
251   ataaacgtat ctattgaata ttattgatga tttataatgg cccgaatctc
301   tatctgggtt tttgtatatg aaagaaaaat ggaagaattg gtgtgaattg
351   atttcacatt gataaaagaa tcgaatattc attgatcaaa tcattcactc
401   cacagtccga tagatctctt aaagaacttt ttattcggac gagaataaag
451   atagagtccc gttctacatg tcaatcccgg caacaatgaa acttatagta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details