JRC GMO-Amplicons | EURL GMFF

Record ID:	OR475243.1
Database:	nt	Go to original record
Description:	Hoya lockii chloroplast, complete genome.
Taxon:	Hoya lockii
Original size:	177063 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	53378	53932	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc aggttgagaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatctt tccatcgaaa attcagaaaa gaaaggatga
251   aagatataca tacatattga atactatatc aaatgattaa taccgaccca
301   aatgagtctg tattttttct ataaaaacgt aagaattggt gtgatttgat
351   tctttcttca atgtggaatc gaatattcat tgatcaaaag attcactcca
401   tagtctgtag atcctctttt caagaactgg attaatcgga cgagaataaa
451   gatagagtcc cgttctacat gtcaatgctg gcaacaatgc aattttgagt
501   aagaggaaaa tccgtcgact taaaaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details