JRC GMO-Amplicons | EURL GMFF

Record ID:	CP132244.1
Database:	nt	Go to original record
Description:	Oryza sativa Japonica Group cultivar Nipponbare isolate AGIS-1.0 chromosome 10.
Taxon:	Oryza sativa Japonica Group
Original size:	23931887 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11372103	11372695	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccatgttttg agaaaacaag cggttctcga
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ctagcggaac tcccttctaa
251   attagggaaa gaagggcttt cgaaatctaa tacacacgta tagatactgg
301   catagcaaac gattaatcac agaactcata tcataatata ggttctttaa
351   ttctttttta aaatgaaaat aggaatgatt tttttagaat tgttgtgaat
401   ccattccaat cgaatattga gtaatcaaat ccttcaattc atagttttcg
451   aaatcttttt aaaagcggat taatcggacg aggataaaga gagagtccca
501   ttctacatgt caatactgac aacaatgaaa tttctagtaa aaggaaaatc
551   cgtcgacttt ctaagtcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details