JRC GMO-Amplicons | EURL GMFF

Record ID:	KX373122.1
Database:	nt	Go to original record
Description:	Poincianella placida voucher Lewis 2046 (K) tRNA-Leu (trnL), trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Erythrostemon placidus
Original size:	593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	592	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaata agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcttttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatgtatact
301   gaaatattat ttcaattgat tagaccagac agaccccaaa tctctatttt
351   ttaatattta tatgacaaat gaaagatgtg aatagattcc aagttgaaga
401   aagaatcgaa tatttattga tcaaatcatt cattccatga tagtctgata
451   aatcttttga agaactgatt aatcggacga gaataaagat agagtcccat
501   tctacatgtc aataccgaca acaatgaaat ttagagtaag aggaaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details