JRC GMO-Amplicons | EURL GMFF

Record ID:	KX373116.1
Database:	nt	Go to original record
Description:	Caesalpinia rostrata voucher ILC6-5 (PRE) tRNA-Leu (trnL), trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Gelrebia rostrata
Original size:	591 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccagaa agggaaaggg
151   agaataaaaa aaggataggt gcagagactt aacggaagct gttctaacaa
201   atggagttga cgacatttcg tttccttagt aaaggaatcc ttcatcgaaa
251   ctccagaaaa gaaaagatca aggatgaaca tatatacgta tacgtactga
301   aatactattt caattgatta gaccagacag aacccaaatc tctatttttt
351   aatattttta tgacaaatga aagatgtgaa tagatttcaa gtttaagaaa
401   gaatcgaata tttattgatc aaattattca ctccatcata gtctgataga
451   tcttttgaag aactgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa taccgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgacttttga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details