JRC GMO-Amplicons | EURL GMFF

Record ID:	KX373105.1
Database:	nt	Go to original record
Description:	Caesalpinia echinata voucher Filgueiras 3391 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Paubrasilia echinata
Original size:	1097 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	603	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gactttattg gattgagcat tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttccga
151   aagggaaagg gagaataaaa aaaggatagg tgcagagact caacggaagc
201   tgttctaaca aatggagttg acgacatttc gtttcgttag taaaggaatc
251   cttccatcga aactccagaa aagaaaggat caaggatgaa catatatata
301   cgtatacgta ctgaaatact atttcaattg attagaccag acagacccca
351   aatctctatt ttttaatatt tatatgacaa atgaaagatg tgaatagatt
401   ccaagttgaa gaaagaatcg aatatttatt gatcaaatca ttcactccat
451   catagtctga tagatctttt gaagaactga ttaatcggac gagaataaag
501   atagagtccc attctacatg tcaataccga caacaatgaa atttctagta
551   agaggaaaat ccgtcgactt tagaaatcgt ggggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details