JRC GMO-Amplicons | EURL GMFF

Record ID:	KX373103.1
Database:	nt	Go to original record
Description:	Guilandina bonduc voucher Herendeen 9-XII-97-3 (US) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Guilandina bonduc
Original size:	1069 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	589	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcck kggtatggca
51    ccctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatata tatacgtata
301   cgtactgaaa tactatttca attgattaga ccagacagac cccaaattaa
351   atatttatat gacaaatgaa agatgtgaat agattccaag ttgaagaaag
401   aatcgaatat ttattgatca aatcattcac tccatcatag tctgatagat
451   cttttgaaga actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat accgacaaca atgaaattta tagtaagagg aaratccgtc
551   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details