JRC GMO-Amplicons | EURL GMFF

Record ID:	KX147312.1
Database:	nt	Go to original record
Description:	Coccinia grandis voucher M:Sousa and Silber 1 chloroplast, complete genome.
Taxon:	Coccinia grandis
Original size:	157035 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49555	50140	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattcaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aaaagggtag
151   gtgcagagac tcaatggaag ctgttctaac aaatggagtt gactacgttg
201   cgttagtaaa ggaatgaatc cttccatcga aactccagaa acgatgaaag
251   ataaacgtat tacgtactga aatactatat tgattaatga caactcgaat
301   ctctatttta tatatatata tatataatga tatataaaaa tgaacgaatt
351   gttatgaatc gattccaata tctccaagtt gaaaaaagaa tcgaatattc
401   attgatcaaa tcatttactc catcatacat agtctgatag atcttttgaa
451   gaactgatta atcagatgcg aatagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaaaaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details