JRC GMO-Amplicons | EURL GMFF

Record ID:	OY776131.1
Database:	nt	Go to original record
Description:	Galeopsis tetrahit genome assembly, chromosome: 13
Taxon:	Galeopsis tetrahit
Original size:	75357568 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	31867259	31867826	-	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattc taatgaaatt gagccttagt
51    gtgggaaacc tgctaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaac gaaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactcc gttggtagag gaatcctttc tacggaaact
251   tcaaaaagga tgaaggataa acgtatctat cgaatactat atcaaatgat
301   taatgatggc cgaatccgta ttttttaata tgaaaaatag aagaattggt
351   gtgaattgat tctatattga aattgaagaa aaaatcgaat attcattcat
401   caaatcattc actccatagt ccgatagatc ttttaaataa ctgattaatc
451   ggacgagaat aaagatagag tcccattcta catgtcaata ctggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details