JRC GMO-Amplicons | EURL GMFF

Record ID:	OR699044.1
Database:	nt	Go to original record
Description:	Prostanthera wilkieana voucher PW MEEK 4 isolate BMR06637 chloroplast, complete genome.
Taxon:	Prostanthera wilkieana
Original size:	152841 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48025	48595	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg ccctggtaga ggcatctttg catggaaact
251   tccgaaagga tgaaggataa atgtatctat tgaatactat atcattaatg
301   attaatgatg gtccgaatct gtatttgtat tttttaatat gaaaaatgga
351   agaattggtg tgaattgatt ccacattgaa gaaaaaatcg aatattcagt
401   cagcaaatga ttcactccat agtccaatag atcttttaaa gaactgatta
451   atcggacgag aataaagata gagtcccatt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details