JRC GMO-Amplicons | EURL GMFF

Record ID:	OR730804.1
Database:	nt	Go to original record
Description:	Persicaria lapathifolia voucher Shmy1 chloroplast, complete genome.
Taxon:	Persicaria lapathifolia
Original size:	159351 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47195	47787	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tcctgctttc aaaaaggaaa gaaaaaaggg
151   ataggtgcag agactcaatg gaagatgttc taacaaatgg aattgactgt
201   cttgcgttgt aatatgttat aagaattctt cgatctaaac gccaaaaagg
251   atgaagaaga aacctgcata ggtacttaaa tagaaagact atactataat
301   ataataaaaa atacaaaaat attgatgacg aaccgaaatt ttcttttatg
351   aatatgaaaa aaatggaaga atttttttga atcgattcta agttgaagaa
401   agaatcgact attcgtttat taaattaaag catttactcc acagtttggt
451   atatcttttg aagaaccgat ccatcggatg agaatgaaga tagagtccca
501   ttctacgtgt caatcccgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt ataaatcgtg agggttcaag tccctctatc ccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details