JRC GMO-Amplicons | EURL GMFF

Record ID:	OR730803.1
Database:	nt	Go to original record
Description:	Persicaria hastatosagittata voucher Jy1 chloroplast, complete genome.
Taxon:	Persicaria hastatosagittata
Original size:	160749 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48703	49294	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaagaa
101   atgggcaatc ctgagccaac tcctgctttc caaaaggaaa gaaaaagagg
151   ataggtgcag agactcaatg gaagttgttc taacaaatgg aattgactgt
201   cttacgttgt aatatgtgat aagaattttt cgatctaaac tccaaaagtg
251   atgaagaaga aagctatagg tatacgtaaa tagaaagact atactatgat
301   ataataaata aagaatgcaa aaatattgat gacggcccga atttttattt
351   tatgaatatg aaaaaaatgg aagaattttt gtgaatcgat tccaagttga
401   agaaagaatc gaatattcgt ttattaaatt aaagcattta ctccacagtt
451   tggtagatct tttgaagacc catcggatga gaatgaagat agagtcccat
501   tctacgtgtc aatcccgaca ccaatgaaat ttatagtaag aggaaaatcc
551   gtcgacttta taaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details