JRC GMO-Amplicons | EURL GMFF

Record ID:	OR730801.1
Database:	nt	Go to original record
Description:	Persicaria longiseta voucher Chz1 chloroplast, complete genome.
Taxon:	Persicaria longiseta
Original size:	159626 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47556	48149	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   aatgggcaat cctgagccaa ctcctgcttt caaaaaggaa agaaaaaggg
151   gataggtgca gagactcaat ggaagatgtt ctaacaaatg gaattgactg
201   gcttgcgttg taatatgtta taagaattct tcgatctaaa cgccaaaaag
251   gatgaagaag aaacctgcat aggtacttaa atagaaagac tatactatga
301   tataataaaa aatacaaaaa tattgatgac gaaccgaaat tttcttttat
351   gaatatgaaa aaaatggaag aatttttttg aatcgattct aagttgaaga
401   aagaatcgaa tattcgttta ttaaattaaa gcatttactc cacagtttgg
451   tatatctttt gaagaaccga tccatcggat gagaatgaag atagagtccc
501   attctacgtg tcaatcccga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tataaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details