JRC GMO-Amplicons | EURL GMFF

Record ID:	OR689485.1
Database:	nt	Go to original record
Description:	Oxalis corniculata tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Oxalis corniculata
Original size:	563 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	563	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggga
51    aacttactaa gtgagaactt tcaaattcag agaaaccctg gaagtaaaaa
101   agggcaatcc tgagccaaat cctgttttcc gaaaacaaac aaagtttgcg
151   aaagcgagaa taaaaaaaag gataggtgca gagactcaac ggaagctatt
201   ctaaagttta caaatagagt tgactgcgtt gcgttagtaa aaaaatcctt
251   tcctaaaaat tccagaaagg ataaacttat atacatacgt atacgtactg
301   aaagactatc tcaaatgatt aatgatgacc taaatctgta ttatttatca
351   aaaaaatcaa agatttgttg tgaatcgatt ccaaaggaat caaatgataa
401   ttgatcaaat cattcactcc agtgtcagat agatcttttg aaaacccgat
451   agaataaaga tagagtccca ttctacatgt caatattgac aacaatgaaa
501   tttatagtaa aaggaaaatc cgtcgacttt agaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details