JRC GMO-Amplicons | EURL GMFF

Record ID:	OR603942.1
Database:	nt	Go to original record
Description:	Incarvillea compacta chloroplast, complete genome.
Taxon:	Incarvillea compacta
Original size:	150154 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	14584	15144	-	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaatct ttccatggaa actgcagaaa
251   ggatgaagga taaatggatc atttgaatac tctctcaaat gattaatgat
301   ggccccaatc tggatctgta ttttttctat gaaaatggaa gaattggtgt
351   gaatggattc cagattgaag aaagaatcga ctattcattc atcaacccct
401   tcactccaga gtccgatagt tctttgaaag aactgattaa tcggtcgaga
451   ataaagatag agtcccattc tacatgtcaa taccggcaaa aaatgaaagt
501   tctagtaaga ggaaaatccg tcgactttca aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details