JRC GMO-Amplicons | EURL GMFF

Record ID:	KT387722.1
Database:	nt	Go to original record
Description:	Orobanche cernua var. cumana chloroplast, complete genome.
Taxon:	Orobanche cernua var. cumana
Original size:	89851 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	21393	21961	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acttgctaag tgataacttt caaattcaga gaaaccccgg aatcaataaa
101   aagggcaatc ctgagccaaa tcctatttta taaaaaaaag tttataaaat
151   gaaaagggga taggtgcaga gactcaatgg aagttattct aacaaacaga
201   gttgattgcg ttggtagaga aatatttcca ttgtaacttc aaaaaggatg
251   aaggataaac atatctatta aatactatat actatataaa ctgattaatg
301   ataacccgaa cctatatctg tttttttgaa tattaaaaac agaagaattg
351   ttgcgaattt cttttatatt gaagaaagaa tattcattca tcaaatcatt
401   cactccatag tctgataaat tttttaaaaa actgattaat cagacgagaa
451   taaagataga gtcccattct acatgtcaat acatgtcaat actggcaaca
501   attaaattta tagtaagagg aaaatccgtc gattttaaaa atcatgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details