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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: KT907838.1
Database: nt Go to original record
Description: Gentiana piasezkii voucher Favre 301 (KUN) trnL-trnF intergenic spacer region, partial sequence; chloroplast.
Taxon: Gentiana piasezkii
Original size: 807 bp
Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2 526 + 525 bp
Amplicon: 525 bp 
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgattatttt caaattcaga gaaaccttgg aattaataaa
101   aagggcaatc ctgagccaaa tcctattttt taaaaagtaa aaaaaaaaaa
151   aagaaaggct taaaaagaaa taaaggatag gtgcagagac tcaatggaag
201   ctgttctaac aaatggagtt ggtgaagagg ggtatataca gagtgtatca
251   aacgattcac ccacggcgtg tagatttttt catgaacgaa ttaatcggac
301   tagaataaag agagagtccc gttctgcatg tcaatgccga caacaatgaa
351   atttttagta agagaaaaat ccktcractt taaaaatcat gagggttcaa
401   gtccctctat cccccaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
451   nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
501   nntgggttca agtccctcta tcccc
Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.36e-08
primerB 100.0 20 bp 0 0 414 395 2.36e-08
primerB 100.0 20 bp 0 0 525 506 2.36e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.