JRC GMO-Amplicons | EURL GMFF

Record ID:	OR597580.1
Database:	nt	Go to original record
Description:	Cornus hongkongensis subsp. melanotricha isolate organ chloroplast, complete genome.
Taxon:	Cornus hongkongensis subsp. melanotricha
Original size:	157288 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48895	49482	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaggttc
151   ggaaagcgaa aataaaaaaa ggggataggt gcagagactc aatggaagct
201   gttctaacac gtggagttga ctaagttgtg ttgttagaag aatctttttt
251   catcgaaact tcagaaagga tgaaggagaa aactatatac atacgtatac
301   gtactgaaat attatatcaa atgattactg acgacacgaa tccttatttt
351   tttatatgaa aaatcaaaga attgttgtga atcgatttca aattgaagaa
401   agaatccaat attcattgat caaatcattc actccgtagt ctgatatatc
451   ttttgaagaa ctgattaatc gaacgagaat aaagatagag tcccattcta
501   catgtcaata ccgacaacaa tgaaatttat agtaagagga aaatccgtcg
551   attttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details