JRC GMO-Amplicons | EURL GMFF

Record ID:	OR582422.1
Database:	nt	Go to original record
Description:	Musa acuminata subsp. malaccensis chloroplast, complete genome.
Taxon:	Musa acuminata subsp. malaccensis
Original size:	170023 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	131683	132276	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttattttga gaaaacaaag gtttataaaa
151   ctagaattta aaaggatagg tgcagagact caatggaagc tgttctaacg
201   aatggagttg actacgtttt gttggtagca ggaatccgtc tataaaaatt
251   acagaaaaga tggtcctata tacctaatac gtatgtatac atactgccat
301   atcaaacgat taatcatgac ccgaatctat tatattataa ttatatataa
351   ttataatatg aaaaattcag agttattatg aatccatttc aatggaagtt
401   gaaggaagaa tcgaatattc aattatgaaa tcattcattc cagagtttga
451   tagatctttt gaaaaactga ttaatcggac gagaataaag agagagtccc
501   attctacatg tcaataccga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt tttaaatcgt gagggttcaa gtccctctat cccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details