JRC GMO-Amplicons | EURL GMFF

Record ID:	OR567249.1
Database:	nt	Go to original record
Description:	Thymus quinquecostatus chloroplast, complete genome.
Taxon:	Thymus quinquecostatus
Original size:	151855 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46786	47329	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggataggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac tgcgttggta
201   gaggaatctt tccatggaaa ctttataaag gatgaaagat aaacgcatct
251   attgaatact atatcaaatg attaatgttg gcccgaatct atttttttaa
301   tatgaaaatg aaaaaatgga aaaatcggtg tgaatttatt tcacgttgaa
351   gaaaaaatag aatattcatc aactcattaa ctccataatc cgatagatct
401   tttaaagaac tttttaatcg ggcgagaata aagatagagt cccattctac
451   atgctacata tcaataccgg caacaatgaa atttatagta agaggaaaat
501   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details