JRC GMO-Amplicons | EURL GMFF

Record ID:	OR567247.1
Database:	nt	Go to original record
Description:	Herpetospermum pedunculosum chloroplast, complete genome.
Taxon:	Herpetospermum pedunculosum
Original size:	156455 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	74879	75489	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aaaagggtag
151   gtgcagagac tcaatggaag ctgttctaac aaatggagtt gactacgttg
201   cgttagtaaa ggaatgaatc cttccatcga aactccagaa aagatgaaag
251   ataaacgtat tacgtactga aatactattt gattaatgac aacccgaatc
301   ttcatttttt atatatttat atataaatat aaaaaatgat ataaatgata
351   tatataaaaa aaaaaatgaa cgaattggta tgaatcgatt ccaatatcgc
401   caagttgaaa aagaatcaaa tattcattga tcaaatcatt tactccatca
451   tacatagtct gatagatctt ttgaagaact gattaatcag atgcgaatag
501   aataaagata gagtcccatt ctacatgtca ataccgacaa aaatgaaatt
551   tatagtaaga ggaaaatccg tcgactttta aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details