JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709429.2
Database:	nt	Go to original record
Description:	Dalea nana voucher KL44 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Dalea nana
Original size:	606 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	606	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaaaaaaa aagttaaaaa
151   agtgagaata aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   agttctaata aatggagttg atgacatttc ttttcgtgtt aggaaaggaa
251   tccttccatc gaaattcaaa aaagagaagg ataaacatac atatatatac
301   atgtatgtac tgaaatacta tttcaattga ttaatgaaga ctccaaatct
351   ctatttgtga atatttctat cacaaatgaa ggatgtgaat caaatcaatt
401   ccaagttgaa gaaagaatgg aatattcatt gatcaaatca ttcactccat
451   cctagtctga tagatctttt gaagaaaact tattaattag acgagaataa
501   agatagagtc ccattctaca tgtcaatacc gacaacaatg aaatttatag
551   taagaggaaa atccgtcgac tttagaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details