JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709428.2
Database:	nt	Go to original record
Description:	Panicum virgatum voucher KL43 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Panicum virgatum
Original size:	614 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	614	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtacggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg taatggaact ccctcgaaat
251   tatagaaaga agggctttat acatctaata cacacgtata gatactcaga
301   tagcaaacga ttaatcatag aacccatatc ataatatagg ttctttattt
351   tattttttag aatgaaatta ggaatgatta tgaaatataa aattctgaat
401   tttttttaga attattgtga atccattcca atcgaatatt gagtaatcaa
451   atccttcaat tcattgtttt gagatcttca aaaaagtgga ttaatcgaac
501   gaggataaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttatagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details