JRC GMO-Amplicons | EURL GMFF

Record ID:	OK187475.1
Database:	nt	Go to original record
Description:	Utricularia tricolor voucher SRS106 chloroplast, complete genome.
Taxon:	Utricularia tricolor
Original size:	149830 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46281	46848	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaacccccgg aataaaaaaa
101   tgggcaatcc tgagccaaat cctgttttct taaaagaaag gttcataaac
151   agaaaaaaaa aaggataggt ccagagactc aatggaagtt gttctaggaa
201   atgggggcgg aatctttcca ccgaaacgtg agaaaagatc aaagataaac
251   ctacctattg aatactatat caaacgagta atcatggcct gaatctctag
301   atgtattatt tttatatgaa aaaaaaaaaa gatatatgaa aaataaaaga
351   attggtgtga attccttcgc cattgaagca aaactagaat ttttattcat
401   caaatcattc actccctagt ccgataaacc ttttcaagaa ctgatcaatt
451   ggacaagaat aaagatagag tcctctccga catgtcaatg ccgacaacaa
501   tgaaatttat agtaagagga aaatccgttg acttttaaaa tcatgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details