JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ935426.1
Database:	nt	Go to original record
Description:	Lolium arundinaceum cultivar Resolute chloroplast, complete genome.
Taxon:	Lolium arundinaceum
Original size:	135324 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46382	46999	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg aggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcgat ggaagctgtt
201   ctaacgaatc gggtaattac gttgtgttgt tagtggaatt ccttctaaat
251   ttgagaaaga agggctttat acatctaata aacacgtata gatactggca
301   tagcaaacga ttaatcatgg aacccatatt ataatatagg ttctttattc
351   ttttttagaa tgcaatttta aacagaaatg attatgaaat caaaaattct
401   gaattttttt tggaattatt gtgaatccat tccaattgaa tattttgtaa
451   tcaaatcctt caattcaaag ttttgaggtc tttaaaaaag tggattaatc
501   ggacgaggac aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttat agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details