JRC GMO-Amplicons | EURL GMFF

Record ID:	OR521089.1
Database:	nt	Go to original record
Description:	Meconopsis pinnatifolia chloroplast, complete genome.
Taxon:	Meconopsis pinnatifolia
Original size:	153557 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46794	47384	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaagggattt ctgaaagcga
151   gaataaaaaa aggataggtg cagagactca atggaagctg ttctaatgaa
201   tggagttgac tgcgttggta gaggaatcct tctattaaat tttaatttaa
251   actccataaa gaatgaggga tgaacctata tacgatacgt acgtatacgt
301   actgaaatat caaagattct attaatgccg ccctaaatat atatttttta
351   cataaaaaag aggcataatt tttgtgaatc tattccaagt tgaaggaaga
401   attgaaaatt cagtgatcaa accattcact ccatagtctg atagatcttt
451   tgacgaactg attaatcgga cgagaataaa gatagagtcc cgttctacat
501   gtcaatatca ataccgacaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgactttat aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details