JRC GMO-Amplicons | EURL GMFF

Record ID:	OR526424.1
Database:	nt	Go to original record
Description:	Tricyrtis xianjuensis chloroplast, complete genome.
Taxon:	Tricyrtis xianjuensis
Original size:	155748 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47821	48424	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaagta aattgagcct tagtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga taaaacaagg atttaattta
151   tcaaactaga ataaaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacgaa tgaagtttat tacgttacgt tggtagctgg aatccttcta
251   tcaaaattac ggagaggatg cctgtatacg tatatataca tactgacata
301   gtaaacaatt gattattgat taatcattga ttaatcgcgg cccaaatcca
351   ttatatatat atgaaaaaat ttagagttat tgtgaatctc ttccaatcga
401   aattgaagga agaatcaaat attcatattc agtgatcaaa ttattcattc
451   cagagtctga tagatctttt gaaaaacgga ttattcggac gagaataaag
501   agagagtccc attttacgtg tgaataccaa caacaatgaa atttatagta
551   aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa gtccctctat
601   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details