JRC GMO-Amplicons | EURL GMFF

Record ID:	AH015556.2
Database:	nt	Go to original record
Description:	Poa wolfii voucher Soreng 5800 US trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa wolfii
Original size:	1873 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	851	1469	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgaaaaag aagggcttta tacatctaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacccatat tataatatag gttctttttt
351   agaatgaaat ttgaaataga aatgattatg aaataaagaa ataaaaaatt
401   cagaattttt ttagaattat tgtgaatcca ttccaatcga atatttagta
451   atcaaatcct tcaattcaaa gttttgaggt ctttaaaaaa gtggattaat
501   cggacgagga caaagagaga gtcccattct acatgtcaat actgacaaca
551   atgaaatttc tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details