JRC GMO-Amplicons | EURL GMFF

Record ID:	KM592170.1
Database:	nt	Go to original record
Description:	Tarenna nitidula voucher Jongkind 8000 (BR) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tarenna nitidula
Original size:	1663 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	734	1299	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcattagtag agaaatcttt catctaaaat tccgaaaggg
251   taaagtgaag gataaacgta tatatatgta ttgaatacta tatcaaatga
301   ttaatgacga ctcgactgaa tctgtatttt ttatatgaaa acggaagaat
351   tggtgtgaat agattacaca ttgaagaaag aatcgaatat tcattgatca
401   aatgattcac tccatagtct gatagatctt ttcaagaatt gattaatcgg
451   acgagaataa agatagagtc ctgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details