JRC GMO-Amplicons | EURL GMFF

Record ID:	KM592153.1
Database:	nt	Go to original record
Description:	Rutidea olenotricha voucher Schmidt & al 1731 (MO) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Rutidea olenotricha
Original size:	1666 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	746	1316	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   gtaaagtgaa ggataaacgt atatatacgt attgaatact atatcaaaca
301   aatgattaat gacgactcga ctgaatctgt attttttata tgaaaacgga
351   agaattggtg ggaatagatt ccacattgaa gaaagaatcg aatattcatt
401   gatcaaatga ttcactccat agtctgatag atcttttcaa gaattaatta
451   atcggacgag aataaagata gagtcctgtt ctacatgtca atgtcggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details