JRC GMO-Amplicons | EURL GMFF

Record ID:	KM592125.1
Database:	nt	Go to original record
Description:	Nichallea soyauxii voucher Dessein & al 1402 (BR) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Nichallea soyauxii
Original size:	1703 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	767	1344	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   gtaaagtgaa ggataaacgt atatatacgt tatatatacg tattgaatac
301   tatatcaaat gattaatgac gactcgactg aatctgtatt ttttatatga
351   aaacggaaga attggtgtga atagattcca cattgaagaa agaatcgaat
401   attcattgat caaatgattc actccacagt ctgatagatc ttttcaagaa
451   tttattaatc ggacgagaat aaagatagag tcctgttcta catgtcaatg
501   tcggcaacaa tgaaatttat agtaagagga aaatccgtcg actttaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details