JRC GMO-Amplicons | EURL GMFF

Record ID:	KT220692.1
Database:	nt	Go to original record
Description:	Perilla setoyensis chloroplast, complete genome.
Taxon:	none
Original size:	152607 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47021	47585	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tgttttctca aaacaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgattgca ctgcactggt agaggaatct ttccctggaa
251   actttagaaa ggatgaagga taaacgcatc tagtgaatac tatatcaaat
301   gattaatgat gggcccgaat ctgttttttt tttaatatga aaaatggaaa
351   aatttgtgtg aattgattcc acgttgaaga aaaaattgaa tattcatcac
401   ctcattcact ccatagtccg atagttcttt taaagaactg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details