JRC GMO-Amplicons | EURL GMFF

Record ID:	KT220685.1
Database:	nt	Go to original record
Description:	Perilla frutescens var. acuta voucher P177 chloroplast, complete genome.
Taxon:	Perilla frutescens var. acuta
Original size:	152588 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47005	47570	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tgttttctca aaacaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgattgca ctgcactggt agaggaatct ttccctggaa
251   actttagaaa ggatgaagga taaacgcatc tagtgaatac tatatcaaat
301   gattaatgat gggcccgaat ctgttttttt ttttaatatg aaaaatggaa
351   aaatttgtgt gaattgattc cacgttgaag aaaaaattga atattcatca
401   cctcattcac tccatagtcc gatagttctt ttaaagaact gattaatcgg
451   acgagaataa agatagagtc ccattctaca tgtcaatacc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details