JRC GMO-Amplicons | EURL GMFF

Record ID:	KM522848.1
Database:	nt	Go to original record
Description:	Diospyros sp. LHM-2015 chloroplast, complete genome.
Taxon:	Diospyros sp. LHM-2015
Original size:	157321 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49405	49990	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgattt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaatcct tccatcgaaa
251   ttttcgaaag gatgaatgag aaacctatat acatacgcat acgtactgaa
301   atactatagc aaacgattaa tgacgaccca aatctgtatg cgtatttttt
351   ttatatgaaa atggaagaat tgttgcgaat cgattcgaca ttgaataaag
401   aattgactat tcattgatca aatcatttac tccatagtct gatagatctt
451   ttgaagaact aattaatcgg acgagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details