JRC GMO-Amplicons | EURL GMFF

Record ID:	KT182634.2
Database:	nt	Go to original record
Description:	Crescentia cujete chloroplast, partial genome.
Taxon:	Crescentia cujete
Original size:	154662 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47631	48203	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg gctgcgttgg tagaggaatc tttccatcga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatatcaaa tgattaatga
301   tagcccgaat ctgtatctgt atttttttgt atttttttat atgaaaaatg
351   gaagaattag tttgaattga ttccacattg aagaaagaat cgaatattca
401   ttcatcaaat cattcactcc atagtccgat agttctttta aagaactgat
451   taagcggacg agaataaaga tagagtccca ttctacatgt caataccggc
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details