JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366206.1
Database:	nt	Go to original record
Description:	Markea epifita isolate LB3264 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Markea epifita
Original size:	1722 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	732	1309	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt cagaaaacaa acaaaggttc
151   agaaaaaaag gaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaaatggag ttaaatgcgt tggtagagga atctttacat cgaaacttca
251   gaaagaaaaa gaatgaagtg aaggataaac gtatatacat acgtattgaa
301   tactatatca aatgattaat gacgacccga atccgtattt tttctataaa
351   aaatagaaga attggtgtga atcgattctc cattgaagaa agaattgaag
401   attcattgat caaatcattc actccatagt ctgatagatc ttttgaagaa
451   ctgattaatc ggacgagaat aaagatagag tcccgttcta catgccaata
501   ccggcaacaa tgcaatttat agtaagagga aaatccgtcg actttaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details