JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366202.1
Database:	nt	Go to original record
Description:	Juanulloa globifera isolate LB3282 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Juanulloa globifera
Original size:	1696 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	730	1282	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttta ctttacatcg aaacttcaga
251   aagaaaaaga atgaagtgaa ggataaacgt atatacatac gtattgaata
301   ctatatcaaa tgattaatga cgacccaaat ccgtattttt tatataaaaa
351   atagaagaat tggtgtgaat cgaatattca ttgatcaaat cattcactcc
401   atagtctgat agatcttttg aagaactgat taatcggacg agaataaaga
451   tagagtcccg ttctacatgt caataccggc aacaatgaaa tttatagtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details