JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366201.1
Database:	nt	Go to original record
Description:	Schultesianthus odoriferus isolate LB3272 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Schultesianthus odoriferus
Original size:	1732 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	742	1301	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   agaattaaca aaaaagggca atcctgagcc aaatcctgtt ttctgaaaac
151   aaacaaaggt tcagaaaaaa aggataggtg cagagactca atggaagcta
201   ttctaacaaa tggagttaaa tgcgttggta gaggaatctt tacatcgaaa
251   cttcagaaag aaaaagaatg aagtgaagga taaacgtata tacatacgta
301   ttgaatacta tatcaaatga ttaatgacga cccgaatccg tattttttct
351   ataaaaaata gaagaattgg tgtgaatcga atattcattg atcaaatcat
401   tcactccata gtctgataga tcttttgaag aactgattaa tcggacgaga
451   ataaagatag agtcccgttc tacatgtcaa taccggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details