JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366190.1
Database:	nt	Go to original record
Description:	Trianaea aff. nobilis GAW-2016 isolate LB3324 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Trianaea aff. nobilis GAW-2016
Original size:	1710 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	718	1296	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaac ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttaa catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaaatgatt aattgattaa tgacgacccg aatccgtatt ttttctataa
351   aaaatagaag aattggtgtg aatcgattct acattgaaga aagaatcgaa
401   tattcattga tcaaatcatt cactccatag tctgatagat cttttgaaga
451   acttattaat cggacgagaa taaagataga gtcccgttct acatgtcaat
501   accggcaaca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details