JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366183.1
Database:	nt	Go to original record
Description:	Markea antioquiensis isolate LB3263 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Markea antioquiensis
Original size:	1715 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	730	1301	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaaa ggataggtgc agagactcaa cggaggctat tctaacaaat
201   ggaggtaaat gcgttggtag aggaatcttt acatcgaaac ttcagaaaga
251   aaaagaatga agggaaggat aaacgtatat acatacgtat tgaatactat
301   atcaaatgat taatgacgac ccgaatccgt attttttcta taaaaaatag
351   aagaattggt gtgaatcgat tctacattga agaaagaatc gaatattcgt
401   tgatcaaatc attcactcca cagtctgata gatcttttga agaactgatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details