JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366177.1
Database:	nt	Go to original record
Description:	Markea panamensis isolate LB3260 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Markea panamensis
Original size:	1727 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	748	1314	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acatcagaca
151   aaaaggatag gtgcagagac tcaacggagg ctattctaac aaatggagtt
201   aaatgcgttg gtagaggaat ctttacatcg aaacttcaga aagaaaaaga
251   atgaagggaa ggataaacgt atatacatac gtattgaata ctctatcaaa
301   tgattaatga cgacccgaat ccttattttt tctataaaaa atagaagaat
351   tggtgtgaat cgattctaca ttgaagaaag aatcgaatat tcgttgatca
401   aatcattcac tcccatagtc tgatagatct tttgaagaac tgattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatac cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details