JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366176.1
Database:	nt	Go to original record
Description:	Juanulloa ochracea isolate LB3323 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Juanulloa ochracea
Original size:	1694 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	714	1285	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agacaaaaag gataggtgca gagactcaac ggaggctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttta catcgaaact tcagaaagaa
251   aaagaatgaa gggaaggata aacgtatata catacgtatt gaatactcta
301   tcaaatgatt aatgacgacc cgaatccgta ttttttctat aaaaaataga
351   agaattggtg tgaatcgatt ctacattgaa gaaagaatcg aatattcgtt
401   aatcaaatca ttcactccca tagtctgata gatcttttga agaactgatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details