JRC GMO-Amplicons | EURL GMFF

Record ID:	KT366159.1
Database:	nt	Go to original record
Description:	Dyssochroma viridiflora isolate LB3327 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Dyssochroma viridiflora
Original size:	1598 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	711	1281	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaac ggaggctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttta catcgaaact tcagaaagaa
251   aaagaatgaa gggaaggata aacgtatata catacgtatt gaatactcta
301   tcaaatgatt aatgacgacc cgaatccgta ttttttctat aaaaaataga
351   agaattggtg tgaatcgatt ctacattgaa gaaagaatcg aatattcgtt
401   gatcaaatca ttcactccat agtctgatag atcttttgaa gaactgatta
451   atcggacgag aataaagata gagtcccgtt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details