JRC GMO-Amplicons | EURL GMFF

Record ID:	OR478166.1
Database:	nt	Go to original record
Description:	Ocimum basilicum var. purpurascens voucher SRMH000147 chloroplast, complete genome.
Taxon:	Ocimum basilicum var. purpurascens
Original size:	152407 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46959	47507	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aagttcaaaa
151   aacgaaaaaa aaaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg gagttgactg cgctggtaga ggaatctttc catggaaact
251   ttcgaaagga tgaaggataa acgcatctat tgaatactat atcaaatggt
301   taatgatggc ccaaatcttt taatatgaaa aaatggaaaa attggtgtga
351   attgattcta cgttgaagaa aaaatcaaat attcatcaac tcattcgctc
401   catagtccga tagataaaga actgattaat cggacgagaa taaagataga
451   gtcccattct acatgtcaat atcggcaaca atgaaattta tagtaagagg
501   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details