JRC GMO-Amplicons | EURL GMFF

Record ID:	OR471583.1
Database:	nt	Go to original record
Description:	Bistorta sinomontana chloroplast, complete genome.
Taxon:	Bistorta sinomontana
Original size:	159795 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48074	48676	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aatttaaaac
101   acgggcaatc ctgagccaac tcctgctttc caaaaaggaa gaataaaaaa
151   ggataggtgc agagactcaa tggaagctgt tctaacaaat ggagttgtct
201   gtcatgcatt gtgctatgtt atacgaattc ttacatctaa actccaaaaa
251   ggatgaagaa gaaacctata ggcatacgta cttaatatct taatatataa
301   aatagaattt atttataata tatctaagat aaaataaaaa ttgatgacga
351   cccgaatctt tattttatga atatgaacaa atggaagaat tattatgaat
401   cgattccgcg ttgaagaaag aatcgaatat tcgtttctta aattaaagca
451   tttactccac agtctgatag atctaccgat ccatcggatg agaatgaaga
501   tagagtccca ttctacgtgt cactcccgac aacaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt taaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details