JRC GMO-Amplicons | EURL GMFF

Record ID:	OM674722.1
Database:	nt	Go to original record
Description:	Hydrocotyle sibthorpioides cultivar Variegata chloroplast, complete genome.
Taxon:	Hydrocotyle sibthorpioides
Original size:	152659 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47834	48422	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa ataacggtgc
151   agaaggcgaa aaggggatag gtgcagagac tcgatggaag ctgttctaac
201   agatgaagtg gactgtgttg gattgataga ggaatccttc cattgaaact
251   tccaaaagga tgaaggataa acgtatatac atacgtatac gtactgaaaa
301   actctatcaa acgattaatg acgaccctaa tctttctttt ttatataaaa
351   acggaagaat tgttgtgaat cgattccata ttgacgaaag aatcgagtat
401   tcattgatca aatcattcac tccataccat atagtctgat atctgataat
451   tcttttgaag aattgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa taccggcaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details