JRC GMO-Amplicons | EURL GMFF

Record ID:	KU523578.1
Database:	nt	Go to original record
Description:	Shibataea kumasaca voucher ISC<USA-IA>:Clark 1290 plastid, complete genome.
Taxon:	Shibataea kumasaca
Original size:	139717 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49190	49802	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagt ggttctcgaa
151   ctagaatcca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttgattac gttgtgttgg tagtggaact ctctctaaat
251   ttagagaaag taagggcttt atacatctaa tacacacgta tagatactga
301   catagcaaac gattaatcac ggaacccata tcataatata ggttctttat
351   tcttttttag aatgaaatta ggaatgatta tgaaatagaa aattctgaat
401   tttttagaat tattgtgaac ccattccaat cgaatattga gtaatcaaat
451   ccttcaattc atagttttcg agatctttta aaaagtggat taatcggacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagttgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details