JRC GMO-Amplicons | EURL GMFF

Record ID:	KU763517.1
Database:	nt	Go to original record
Description:	Poa pauciflora voucher Peterson et al. 21777 US trnT-trnF intergenic spacer region, partial sequence; chloroplast.
Taxon:	Poa pauciflora
Original size:	1784 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	816	1434	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagnccaaat ccgtgttttg agaaaacaag ggggttctcg
151   aactagaata caaaggaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacgaat cgagttaatt acgttgtgtt gttagtggaa ttccttcgaa
251   attctaaaaa gaagggcttt atacagctaa taaacacgta tagatactga
301   gatagcaaac gattaatcac agagcccata ttagaatata atattggttc
351   tttattcttt tttagaatga aatttgaaat agaaatgatt atgaaataaa
401   aaattcataa tttttttaga attattgtga atccaatcga atatttagta
451   atcaaatcct tcagttcaaa gttttgaggt ctttaaaaaa gtggattaat
501   cggacgagga caaagagaga gtcccattct acatgtcaat actgacaaca
551   atgaaatttc tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details