JRC GMO-Amplicons | EURL GMFF

Record ID:	KU763510.1
Database:	nt	Go to original record
Description:	Poa mendocina voucher Peterson et al. 19202 US trnT-trnF intergenic spacer region, partial sequence; chloroplast.
Taxon:	Poa mendocina
Original size:	1790 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	828	1439	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagnccaaat ccgtgttttg agnnnnnaag ggggttctcg
151   aactagaata caaaggaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacgaat cgagttaatt acgttgtgtt gttagtggaa ttccttcgaa
251   attctaaaaa gaagggcttt atacagctaa taaacacgta tagatactga
301   gatagcaaac gattaatcac agaacccata ttataatatt ggttctttat
351   tcttttttag aatgaaatag aaatgattat gaaataaaaa attcataatt
401   tttttagaat tattgtgaat ccattccaat cgaatattta gtaatcaaat
451   ccttcaattc aaagttttga ggtcgttaaa aaagtggatt aatcggatga
501   ggacaaagag agagtcccat tctacatgtc aatactgaca acaatgaaat
551   ttctagtaaa aggaaaatcc gtcgacttta taagtcgtga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details