JRC GMO-Amplicons | EURL GMFF

Record ID:	KT428154.1
Database:	nt	Go to original record
Description:	Scrophularia dentata voucher XZ201406 chloroplast, complete genome.
Taxon:	Scrophularia dentata
Original size:	152553 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47487	48050	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   ggcgaaaaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttgactg cgttggtaga gaaatctttc catcgaaact tcagaaaggg
251   tgacgaataa acgtatctat tgaatactat atcaaatgat taatgatggc
301   ccgaatcttt atctttattt ttttatatga aaaatagaaa aattgatgtg
351   aatttattcc acattgaaga aagaatcaaa taaaatattc attcatcaaa
401   tcattcactc catagtctga tagttctttt aaagaactga ttaatcggac
451   gagaataaag atagagtccc attctacatg tcaataccgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details